1. Give examples of two types of RNA that are not used to make protein.

2. Sequence relationships:

1. If an RNA molecule has the sequence 5’AGGCCU3′, give the sequence of the template and nontemplate DNA strands.
2. If part of a DNA template strand has the sequence 5’TTCGAA3’, give the sequence of the RNA and nontemplate DNA strands.
3. If part of a DNA nontemplate strand has the sequence 5’CCTGAG3’, give the sequence of the RNA and the template DNA strands

3. Compare and contrast the processes of replication and transcription: Create a table that could be used as a study tool. In your table, be sure to include the chemical composition of DNA and RNA, the biochemistry of DNA and RNA synthesis, and the templates and the products of the reaction including the parts of the genome used.

4. Draw a diagram of a transcription bubble. Label 5’ and 3’ ends of the template, nontemplate, and RNA strands, and indicate the direction of transcription.

5. A simplified gene sequence is diagrammed below. The underlined sequences are, in order, the -35, -10, +1, and terminator sequences.

5’aaTTGACAactgtgtgatgtagcttaTATAATatgatgctaCgttaaaggaggtgggtaatgcctaaatggcggtaacgtgaGGCCGCGGCCTTTTcccc3’

1. What role does each of these underlined elements play in transcription?
2. Is this the template strand or nontemplate strand of the DNA?
3. What is the sequence of the RNA that is produced from this gene?
4. Is this a eukaryotic or prokaryotic gene?

6. Draw a diagram of a prokaryotic gene and the DNA elements necessary for transcription. Include the -10 and -35 boxes, the site at which transcription begins, and the site at which transcription ends.

7. Draw a diagram of a eukaryotic gene with two introns. Include the DNA elements necessary for transcription and RNA processing. Include the 5’ and 3’ splice sites, the TATA box, a CAAT box, the polyadenylation signal, the start site of transcription, and the approximate location where transcription would end.

8. Here are some links to additional practice questions for splicing:

1. 1. Diagram the meg-1 gene.
   2. Alternative splicing of the egl-15 gene

Science and Society

9. Choose one of the researchers mentioned in the modules on Replication or Transcription. Ask ChatGPT or another generative AI to write a 500-1000 word biography about them.  Repeat this for the same researcher multiple times.  What common themes appear?  How does that researcher’s identity and background appear to influence their work?